If you like us, please share us on social media.
The latest UCD Hyperlibrary newsletter is now complete, check it out.
Copyright (c) 2006-2014 MindTouch Inc.
This file and accompanying files are licensed under the MindTouch Master Subscription Agreement (MSA).
At any time, you shall not, directly or indirectly: (i) sublicense, resell, rent, lease, distribute, market, commercialize or otherwise transfer rights or usage to: (a) the Software, (b) any modified version or derivative work of the Software created by you or for you, or (c) MindTouch Open Source (which includes all non-supported versions of MindTouch-developed software), for any purpose including timesharing or service bureau purposes; (ii) remove or alter any copyright, trademark or proprietary notice in the Software; (iii) transfer, use or export the Software in violation of any applicable laws or regulations of any government or governmental agency; (iv) use or run on any of your hardware, or have deployed for use, any production version of MindTouch Open Source; (v) use any of the Support Services, Error corrections, Updates or Upgrades, for the MindTouch Open Source software or for any Server for which Support Services are not then purchased as provided hereunder; or (vi) reverse engineer, decompile or modify any encrypted or encoded portion of the Software.
A complete copy of the MSA is available at http://www.mindtouch.com/msa
When the indicator is initially added to the solution, the solution turns blue. The color gradually fades and becomes colorless. The color reappears when the flask is vigorously shaken. The color then begins to fade again.
The color change results from the reversible oxidation-reduction reaction of the methylene blue indicator. In alkaline solutions, glucose is oxidized to D-gluconic acid or alpha-D-gluconolactone
HOCH2(CHOH)4CHO + 3 OH - ---> HOCH2(CHOH)4CO2 + 2 H2O + 2 e -
In the course of this reaction, methylene blue is reduced from the blue (oxidized) form to the colorless (reduced) form.
Shaking the flask dissolves O2 in the solution, which oxidizes the indicator back to the blue (oxidized) form.
An NSF funded Project